As the leading pole of China's future economic development, Guangdong-Hong Kong-Macao Greater Bay Area's urbanization process has had a significant impact on its landscape pattern, which has affected the changes in ecosystem services. Therefore, studying the landscape pattern change of Guangdong-Hong Kong-Macao Greater Bay Area and its impact on the changes of ecosystem services is of great significance to its ecological, economic and social sustainable development. Based on Fragstats 4.2 software and InVEST model, this paper discussed the landscape pattern and ecosystem service changes in Guangdong-Hong Kong-Macao Greater Bay Area from 2000 to 2015, and analyzed the correlation between landscape index(PD, LPI, ENN_MN, SHDI, CONTAG, AI)and ecosystem services (carbon storage,soil retention,habitat quality). The results show that the landscape pattern of Guangdong-Hong Kong-Macao Greater Bay Area has undergone significant changes from 2000 to 2015, and the fragmentation has intensified,and the spatial heterogeneity of the landscape has been continuously enhanced. Affected by changes in landscape patterns, carbon stocks in Guangdong-Hong Kong-Macao Greater Bay Area showed an upward trend from 2000 to 2015, while soil retention capacity and habitat quality showed a downward trend, and various ecosystem services were heterogeneous in space. The changes of woodland, cropland and built-up land have great influence on the landscape pattern and ecosystem services of Guangdong-Hong Kong-Macao Greater Bay Area. Therefore, optimizing the land use structure between three landscapes is the key to the sustainable development of regional ecological protection and economic development.