Cellular energy supply is a key factor in controlling senescence and disease while the senescence and disease of postharvest horticultural crops may be attributed to limited availability of energy or low energy generation. The paper reviewed the recent advance in energy generation and control and then proposed the regulation mechanism of energy availability based on the synthesis, translocation and dissipation of harvested horticultural crops.

Silver staining is an effective method to detect SSRs with high resolution in polyacrylamide gel. However the conventional methods of silver staining are time-consuming because they have a number of time-dependent procedures with more kinds of reagents. Here we reported a rapid and effective silver staining method for detecting PCR amplification products in nondenaturing polyacrylamide gel by using SSRs of flowering Chinese cabbage and tobacco. This method includs two major steps——staining and development,within 6 to 7 mins, and requirs only three reagents which are HNO₃, NaOH and HCOH. With the method, DNA fragments of SSRs are unambiguously detected with high resolution. Compared with other silver staining methods, this new method has advantages of easy operation, time-saving, less amount of chemical reagents and good detection effect.

Existence of solutions to the boundary value problems of a 2mth-order difference equation is considered in this paper. By establishing the corresponding variational structure, we transform the problem of the existence of solutions to the boundary value problems into the existence of the critical points for the corresponding variational functional. By using Linking Theorem, we obtain the existence of the critical points of the variational functional, and the existence of solutions of the boundary value problems is achieved as well. An illustrative example is presented at length to illustrate our conclusion.

In this paper, we study some properties of Toeplitz operators on the Bergman-Sobolev space. Mainly, we calculate the essential norm of these operators in terms of the boundary value of their corresponding symbols.

The structure of radio emission beam of pulsars is a problem of long-term debate. The conal beam and the fan beam models are currently the two models that can make explicit predictions. They give the opposite predictions on the relationship between the pulse width and the impact angle between the line of sight and the magnetic axis of pulsar. In this paper, a sample of 85 pulsars with known pulse width and impact angle was collected from literature and used to test the model predictions. It is hitherto the largest sample for the model test. It is shown that the relationship between the pulse width and the impact angle predicted by the fan beam model is well consistent with the observational data, while that of the conal beam model is significantly inconsistent with the data. Our result poses a further challenge to the conal beam mode, which is currently the prevailing emission beam model.

This paper aims to study the effects of alkyl polyglucosides (APG) aqueous solution concentration, sodium chloride and polyethylene glycol on the rheological properties of APG and its complex systems. The results show that different rheological characteristics are found in different concentrations of APG solution. APG is non-Newtonian fluid when the concentration is≥9%, and APG is Newtonian fluid when the concentration is 8%. The effect of salt concentration on APG rheological properties is significant. The solution shifts from non-Newtonian fluid to Newtonian fluid when the NaCl concentration is ≥1%. The rheological behavior of polyethylene glycol and APG complex system is changed. The complex system is Newtonian fluid when polyethylene glycol concentration was low, and the complex system is non-Newtonian fluid when polyethylene glycol concentration is high.

The species community diversity of Disanthus cercidifolius var. longipes in 6 plots from Jinggang Mountain, Guanshan Mountain, Junfeng Mountain, Shanqing Mountain in Jiangxi Province and Lianhua Mountain in Zhejiang Province were studied. The result shows that the community floristic composition is abundant. There are 283 species belonging to 170 genera and 80 families. The species in the tree layer is simplex, and the dominant species is prominent. D. cercidifolius var. longipes and Rhododendron latoucheae are dominant species in the shrub layer. Simpson index range is about 0.443 6~0.949 2, Shannon-Wiener index range is about 1.459 0~3.642 2 and Pielou evenness index range is about 0.359 3~0.759 8 of the six communities with D. cercidifolius var. longipes. Guanshan Mountain Plot has the highest indexes, while Jinggang Mountain Plot has the lowest. On the average, the species richness index, the species diversity index and the species evenness index are in the order of shrub layer > tree layer. Frequency analysis indicates that the frequency spectra of the communities does not comply with the law of Raunkiaer standard frequency. Only two quadrats from Guanshan Mountain are similar in the shape of the graph. All the communities are subject to artificial destruction. The protection should be strengthened with some measures, such as in situ conservation and artificial cultivation, etc.

This essay presented cross imaging algorithm based on digital image technology and the image resolution was improved by crossing calculation of images captured by two thermal infrared imaging cameras. The ship at sea works as a target object. By separating the images, sea level mapping, and ship identification, high precision positioning of the target object can be achieved. Its accuracy of measurement appropriately satisfies the needs of projects. Thermal infrared images with high precision play important roles in project monitoring and measurement. This also expands the application of infrared thermal imaging technology in actual project monitoring and measurement.

Traditionally, the wind-induced vibration of a tall building can be categorized into two parts, the background response and the resonant response, and the total response is then computed by the SRSS (Square Root of the Sum of the Squares) method. This treatment helps understand the mechanism of wind-induced vibration for a tall building at the expense of computational errors. In this study, a super-tall building, the Guangzhou East Tower with a height of 530 m is sampled to show the relative error between the results by the accurate method and those obtained from the approximate method. The wind tunnel test is carried out by adopting the high-frequency force balance technique, and some wind-induced responses such as base overturning moment response, displacement response and acceleration response atop the building. The correlations between the background response and the resonant response are analyzed. The results indicate that for the Guangzhou East Tower, the correlation coefficients vary mainly in the range of -10% to 10% under most wind directions, however in some particular wind direction at which the maximum response occurs, the correlation coefficients are usually large. The maximum correlation coefficient is around 86%. Neglecting the correlation between the background response and the resonant response could cause overestimation or underestimation of the structural response. It is suggested that the accurate method be adopted directly to compute the wind-induced response of super-tall buildings.

In order to obtain the time history of 3dimensional fluctuating wind speed with the same statistical characteristics as the wind of near ground atmosphere boundary layer, an inflow turbulence generation method called DSRFG (discretizing and synthesizing of random flow generation) is applied to generate the time record of inflow turbulence wind speed for Large Eddy Simulation in CFD, meanwhile an improved dynamic oneequation model is adopted as the subgrid scale model for Large Eddy Simulation method. These two methods are combined for the CFD simulation on the wind load distribution and characteristics of an isolated rectangular tall building with 1∶1∶3 ratio (the ratio between length, width and height) in zero degree wind direction. The CFD numerical simulation results are further compared with those experimental wind tunnel test results on the same building from TPU wind tunnel laboratory in Japan. All these comparisons indicate that: the fluctuating kinetic energy of windinduced torsional moment mainly results from the influence of vortex shedding other than the inlet turbulence for the test building; the combination of DSRFG with the improved dynamic one-equation model in the large eddy simulation method in CFD can accurately obtain the value and their characteristics of mean and fluctuating wind loads acting on the isolated rectangular tall building, which jointly testify the feasibility of its application to the design wind load evaluation for actual high rise buildings.

The digital audio network transmission system in a concert hall of X-provincial opera house is presented in this paper. In accordance with the design goal, the product type is selected, and the design scheme, corresponding equipment arrangement and system diagram are provided. The design can guarantee the synchronization and real-time performance during the audio transmission process, which can realize the routing function of multi-channel audio and digital signal processing.Implementation results shows the goal of the design is achieved.

Simultaneous Wireless Information and Energy Transfer (SWIET) is an efficient technique to solve the energy-constrained problem in wireless communication networks. In this paper, the Simultaneous Information and Energy Transfer in an OFDM amplify-and-forward relay network with an energy-constrained relay is investigated. The goal is to improve the system end-to-end transmission rate and extend the lifetime of the relay’s battery. Firstly, the optimal energy transfer policy and subcarrier pairing scheme is studied. Then, the power allocation of information transmission and energy transfer time ratio optimization problem is formulated as a non-convex problem, where the objective function and the energy-harvesting constraint are both non-convex. By solving the non-convex problem with fractional programming technique and CCCP (Constrained Concave Convex Procedure) method, the resource allocation optimization algorithm is provided. The simulation results show that our proposed resource allocation scheme can significantly improve the rate performance of SWIET-based OFDM relay networks, compared with the existing resource allocation schemes.

Landfill is regarded as one of the main approach for municipal solid waste treatment for its mature technology and economy all over the word. However, the landfill sites will produce leachates which contain abundant organic pollutants and may cause serious pollution to water around the Landfill. Two landfills—Likeng and Xingfeng in Guangzhou city were chosed for investigating. By analyzing the content and distribution of two typical persistent organic pollutants— polycyclic aromatic hydrocarbons and phthalate esters in leachate, surface and underground water around the landfill sites, their pollution characteristics were estimated. The results showed that the concentration of ∑PAHs and ∑ PAEs in all the leachate samples was high and with mean values of 417 μg·L^-1and 180 μg·L^-1 respectively. PAHs and PAEs were detected in all the water samples taken from the Likeng and Xingfeng landfills. The concentration of ∑PAHs was 1.4~2.8 μg·L^-1,with mean value of 2.0 μg·L^-1. The concentration of ∑ PAEs was 1.1~7.4 μg·L^-1,with mean value of 2.4 μg·L^-1. The concentration of ∑PAHs and ∑PAEs in underground water decreased as they moved away from the landfill sites.The similar characteristics of constituents and changes in PAHs and PAEs in surface water, underground water and the leachates indicated that these organic pollutants came from the same source.

The broad area of estuarine and coastal waters is characterized with high biological productivity. It is a typical area ecologically affected by human activities and the terrestrial material. In this paper, the remote sensing inversion model for the concentration of chlorophyll a and suspended matter of coastal waters in the Pearl River estuary is analyzed, and the applicability of the existing remote sensing retrieval model for chlorophyll a concentration and suspended matter concentration in experimental regions is validated. Based on the combination of measured data and remote sensing image data, we adopt statistical analysis methods to establish the retrieval model for chlorophyll a concentration and suspended matter concentration of coastal waters in the Pearl River estuary. Landsat 8 inverseion image is utilized to reflect the distribution of chlorophyll a concentration, surface water quality information and suspended matter concentration in coastal waters of the Pearl River estuary. The inversion results being in accordance with the theoretical analysis and the actual situation, it is proved that the remote sensing retrieval model of water quality parameter established in this study can be applied to water quality monitoring for coastal waters in the Pearl River estuary.

Resource utilization of sewage sludge char should be paid more attention to. This work reported a process for preparation of hollow-like granular sludge char (HGC) using carbon fiber as the core and the sludge char as the shell. In this process, the sludge paste mixed with ZnCl₂ was coated on the spherical cotton surface, and further dried and pyrolyzed at 500 ℃. The pore structure characteristics of the HGC were investigated by nitrogen adsorption-desorption curves and SEM analysis. Further, the HGC was conducted as filler in the BAF for treatment of the effluent from secondary. The performance of the HGC filler in BAF was evaluated by the COD, NH⁺₄-N and T-N removal efficiencies under varied parameters, further being compared with the commercial activated carbon. Results showed that the COD, NH⁺₄-N and T-N removal efficiencies for HGC are higher than those for activated carbon. Thus, it is a promising process for preparation of efficient BAF filler in the treatment of sewage as well as sludge.

Urban expansion links closely with industrialization, economic development and energy consumption. Based on this consideration, decoupling analysis model of urban expansion and energy carbon emissions was first established. Then, comparisons of decoupling analysis of urban expansion and energy carbon emissions in 2001~2010, 2001~2006 and 2006~2010 at the country level, regional level and provincial level were made by using statistical data. Results show that energy carbon emissions increased more rapidly than urban expansion in 2001~2010 at the country level. Energy carbon emission reduction faced serious challenges. Specifically, emission reduction technologies related to energy consumption, energy consumption structure and efficiency had much potential to improve. Pressure of energy carbon emission reduction at the country level, however, reduced from 2001~2006 to 2006~2010. Meanwhile, it varied remarkably among Eastern, Central and Western China and provinces.

Nansha district is an important area of Guangzhou city, which has rich wetland resources. If these wetlands could be reasonably used and protected, it would benefit the sustainable development of Nansha. Based on the Frame Sampling Tool of Erdas Imagine and data of Landsat OLI imagery, the methodology of stratified sampling was applied to remote sensing image classification of the Nansha wetlands. The research results showed that: ①The stratified sampling methodology has a high classification accuracy (an overall accuracy of 84% and a Kappa coefficient of 0.8 were achieved in this study); ②This method has a high efficiency on sample estimating, training and management via Frame Sampling Tool of Erdas Imagine software; ③Nansha district of Guangzhou has rich wetland resources, which occupy 41.05% of the study area, and mainly distribute near the estuary and branches of the Pearl River.